Cre-lox Technology in Mouse Modeling

Self-paced Online MiniCourse · 1 – 2 hours · Digital badge and certificate eligible

Learn how to best use Cre-lox gene editing technology to genetically engineer general, tissue specific and inducible knock-outs, apply the FLP-FRT system and turn on fluorescent reporter genes.

• The P1 bacteriophage viral life cycle
• The basic Cre-lox system
• The three Cre-lox mechanisms
• Breeding schemes for tissue specific, general and inducible knock-outs
• Improving conditional knock-out efficiency
• The FLP-FRT system
• The Cre reporter strain
• Overview of the JAX Cre Repository

This MiniCourse is designed to meet the needs of people who utilize JAX laboratory mice for genetic and genomic research projects, including: scientists, research assistants, lab technicians, graduate and postdoctoral students, mouse colony managers and staff.