Cre-lox Technology in Mouse Modeling

Self-paced JAX Online MiniCourse

Cre-lox Technology in Mouse Modeling

Learn how to best use Cre-lox gene editing technology to genetically engineer general, tissue specific and inducible knock-outs, apply the FLP-FRT system and turn on fluorescent reporter genes.

MiniCourse Overview

This MiniCourse is self-paced and takes 1 to 2 hours to complete. At the end of the MiniCourse, you will be able to understand how the Cre-lox system is applied to make different types of mouse models for research—including tissue specific knock-outs, general knock-outs and inducible knock-outs—or to turn on fluorescent reporter genes. You have the option to purchase a JAX digital badge credential and certificate. In order to purchase and display a JAX digital badge credential and certificate for this MiniCourse, you must complete the Core Lessons and take the Self-Review Quiz (scoring at least 70%).

Topics

The P1 bacteriophage viral life cycle
The basic Cre-lox system
The three Cre-lox mechanisms
Breeding schemes for tissue specific, general and inducible knock-outs
Improving conditional knock-out efficiency
The FLP-FRT system
The Cre reporter strain
Overview of the JAX Cre Repository

Target Audience

This MiniCourse is designed to meet the needs of people who utilize JAX laboratory mice for genetic and genomic research projects, including: scientists, research assistants, lab technicians, graduate and postdoctoral students, mouse colony managers and staff.

About our Education Offerings

As an independent, nonprofit biomedical research institution founded in 1929, The Jackson Laboratory leverages its unique combination of research, education and resources to achieve its mission: to discover precise genomic solutions for disease and empower the global biomedical community in its shared quest to improve human health. We offer comprehensive educational programs for scientists throughout their careers, from high school students and teachers to researchers at all experience levels, and for clinical providers interested in incorporating genetics and genomics into their practices.

While we are not an accredited higher education institution, we offer a variety of education programs — including free non-credit bearing MicroLessons and MiniCourses, with optional digital badge credential and certificate for purchase — designed to educate current and future scientists and to provide critical resources, data, tools and services to researchers worldwide.

MiniCourse topics address both foundational content and the research areas in which The Jackson Laboratory has earned a reputation for scientific leadership and excellence.

Hardware and Software Requirements